Summary
Type: SPE
Application #: 300470
Catalog#: 730079 - SPE Cartridges, Chromabond, SB, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
1 column volume n-hexane, then 1 column volume methanol, finally 1 column
volume dist. water
Sample application:
Slowly force or aspirate the sample through the column
Column washing:
1 column volume dist. water; dry column 3 – 4 min under vacuum
Elution:
2 x 500 μl 0.1 N hydrochloric acid, for acid sensitive nucleotides 2 x 500 μl
0.1 N aqueous sodium carbonate solution
Authors: Macherey-Nagel
Source: LC department